Author: computersciencea95821e0fc
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Newsletter Unsynced pattern
Supply Chain Special Edition! Contact Us Amino Acid and Vitamin Supply Chain Shortage Update The Federal Government and specialized tradegroups like the American Feed Industry Association have released an advisory warning of supply chain distruptions for critical amino acids and vitamins used in cell culture media, feed blending, and biopharma manufacturing. China is the manufacturing…
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Glucosamine Analysis by Hitachi
Purpose Glucosamine (C6H13NO5) is an amino sugar and is part of the structure of polysaccharides, chitosan, and chitin. Glucosamine is one of the most abundant monosaccharides in biological systems. It is produced commercially by the hydrolysis of shellfish exoskeletons or, less commonly, by fermentation of grain such as corn or wheat. Glucosamine in nutrient products…
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Fatty Acid Analysis by Shimadzu GC-2010
Purpose Lipids and fatty acids serve several fundamental physiological roles in nature including the formation of lipid bilayers, as energy substrate and as precursors of eicosanoids. Fatty acid analysis can identify trends in essential fatty acid status that may signify aberrations in physiology and or cell culture systems. Fatty acids in a variety of matrices…
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Total Lipid Analysis by Shimadzu Gas Chromatography
Purpose Total Lipids in a variety of matrices are quantified using a Shimadzu GC analyzer. This method is for accurate determination and quantification of total lipids. Summary The total lipids in a sample are extracted with mixtures of chloroform and methanol. The collected lipid extracts are then trans esterified into fatty acid methyl esters. Fatty…
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Total Hydrolyzed Amino Acid Analysis by HPLC
Purpose Samples containing peptides and proteins can be hydrolyzed to release the bound amino acids. By doing a performic acid pretreatment on the protein, this ensures that the sulfur containing amino acids Cysteine and Methionine are not damaged during the hydrolysis procedure. In order to preserve any Tryptophan, samples are put through an alkaline hydrolysis.…
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Performic Acid Pretreatment and Hydrolyzed Amino Acid Analysis
Purpose Samples containing peptides and proteins can be hydrolyzed to release the bound amino acids. By doing a performic acid pretreatment on the protein, this ensures that the sulfur containing amino acids Cysteine/Cystine and Methionine are not broken during the hydrolysis procedure. This method is for accurate determination and quantification of Cysteine/Cystine and Methionine in…
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Hydrolyzed Amino Acid Analysis by Dedicated HPLC Amino Acid Analyzers
Purpose Samples containing peptides and proteins can be hydrolyzed using an acid stable hydrolysis procedure to release the bound amino acids. This method is for accurate determination and quantification of amino acids in proteins. Summary Samples are digested using an acid-stable hydrolysis procedure to release the bound amino acids present in the protein of the…
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Purified Protein and Monoclonal Antibody Analysis by Dedicated Amino Acid Analyzers
Purpose Samples of purified proteins and monoclonal antibody proteins can be hydrolyzed to release the bound amino acids to accurately determine a concentration of each individual residue. A performic acid pretreatment on the protein ensures that the sulfur containing amino acids Cysteine and Methionine are not broken during the hydrolysis procedure. To preserve any Tryptophan,…
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Free Amino Acid Analysis by Hitachi
Purpose Free amino acids in cell culture media, blood plasma, and other matrices can be separated, identified, and quantified via Hitachi amino acid analyzers. This method is for accurate determination and quantification of amino acids Summary Samples are prepared for analysis by diluting a portion of the sample with a deproteinizing solution (5-sulfosalicylic acid hydrate),…
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Elemental analysis by Inductively Coupled Plasma Mass Spectrometry
Elements and metals in cell culture media can be identified and quantified via Inductively Coupled Plasma Mass Spectrometry. This method outlines the accurate determination and quantification of elements and metals. Summary Samples first undergo a digestion process to extract the elements and metals from the sample matrix. This process is achieved by adding strong acids…
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Collagen Analysis by Hitachi Amino Acid Analyzer
Collagen is the main protein of connective tissue in animals and is the most abundant protein in mammals, making up about 25% of the total protein content. Hydroxyproline, Proline and Glycine make up approximately 50% of collagen. Hydroxyproline and Hydroxylysine are the 2 major cross-linking amino acids in collagen and contribute to collagen’s strength. This…
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Tryptophan analysis
Purpose Samples containing peptides and proteins can be hydrolyzed to release the bound amino acids. In order to preserve Tryptophan, samples are put through an alkaline hydrolysis. This method is for accurate determination and quantification of amino acids in proteins. Summary Samples are prepared using an alkaline hydrolysis method to release and preserve bound Tryptophan…
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Water-soluble vitamins
Purpose Water-soluble vitamins in cell culture and fermentation media can be separated, identified, and quantified via HPLC. This method outlines the accurate determination and quantification of water-soluble vitamins. Summary A portion of liquid sample is placed in an amber HPLC vial after undergoing the appropriate preparation methods. The separation uses a reversed phase C-18 column…