Tag: Feed and Agriculture
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Glucosamine Analysis by Hitachi
Purpose Glucosamine (C6H13NO5) is an amino sugar and is part of the structure of polysaccharides, chitosan, and chitin. Glucosamine is one of the most abundant monosaccharides in biological systems. It is produced commercially by the hydrolysis of shellfish exoskeletons or, less commonly, by fermentation of grain such as corn or wheat. Glucosamine in nutrient products…
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Fatty Acid Analysis by Shimadzu GC-2010
Purpose Lipids and fatty acids serve several fundamental physiological roles in nature including the formation of lipid bilayers, as energy substrate and as precursors of eicosanoids. Fatty acid analysis can identify trends in essential fatty acid status that may signify aberrations in physiology and or cell culture systems. Fatty acids in a variety of matrices…
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Total Lipid Analysis by Shimadzu Gas Chromatography
Purpose Total Lipids in a variety of matrices are quantified using a Shimadzu GC analyzer. This method is for accurate determination and quantification of total lipids. Summary The total lipids in a sample are extracted with mixtures of chloroform and methanol. The collected lipid extracts are then trans esterified into fatty acid methyl esters. Fatty…
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Total Hydrolyzed Amino Acid Analysis by HPLC
Purpose Samples containing peptides and proteins can be hydrolyzed to release the bound amino acids. By doing a performic acid pretreatment on the protein, this ensures that the sulfur containing amino acids Cysteine and Methionine are not damaged during the hydrolysis procedure. In order to preserve any Tryptophan, samples are put through an alkaline hydrolysis.…
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Performic Acid Pretreatment and Hydrolyzed Amino Acid Analysis
Purpose Samples containing peptides and proteins can be hydrolyzed to release the bound amino acids. By doing a performic acid pretreatment on the protein, this ensures that the sulfur containing amino acids Cysteine/Cystine and Methionine are not broken during the hydrolysis procedure. This method is for accurate determination and quantification of Cysteine/Cystine and Methionine in…
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Hydrolyzed Amino Acid Analysis by Dedicated HPLC Amino Acid Analyzers
Purpose Samples containing peptides and proteins can be hydrolyzed using an acid stable hydrolysis procedure to release the bound amino acids. This method is for accurate determination and quantification of amino acids in proteins. Summary Samples are digested using an acid-stable hydrolysis procedure to release the bound amino acids present in the protein of the…
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Free Amino Acid Analysis by Hitachi
Purpose Free amino acids in cell culture media, blood plasma, and other matrices can be separated, identified, and quantified via Hitachi amino acid analyzers. This method is for accurate determination and quantification of amino acids Summary Samples are prepared for analysis by diluting a portion of the sample with a deproteinizing solution (5-sulfosalicylic acid hydrate),…
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Collagen Analysis by Hitachi Amino Acid Analyzer
Collagen is the main protein of connective tissue in animals and is the most abundant protein in mammals, making up about 25% of the total protein content. Hydroxyproline, Proline and Glycine make up approximately 50% of collagen. Hydroxyproline and Hydroxylysine are the 2 major cross-linking amino acids in collagen and contribute to collagen’s strength. This…
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Water-soluble vitamins
Purpose Water-soluble vitamins in cell culture and fermentation media can be separated, identified, and quantified via HPLC. This method outlines the accurate determination and quantification of water-soluble vitamins. Summary A portion of liquid sample is placed in an amber HPLC vial after undergoing the appropriate preparation methods. The separation uses a reversed phase C-18 column…